Pertumbuhan Eksplan Bunga Betina Kelapa Sawit (Elaeis guineensis Jacq.) pada Media MS dengan Kombinasi 2,4D dan BAP

Abstract

The research of “The Effect of 2,4-D and BAP Combination in MS Media on The Growth of The Immature Inflorescences of Oil Palm (Elaeis guineensis Jaqc.)” has been done in Plant Tissue Culture Laboratory, University of Sumatera Utara from January 2013 to December 2013. The aim of this research was to know the effects combination 2,4-D and BAP for callus induction and the growth of the immature inflorescences of oil palm (Elaeis guineensis Jacq.) using Complete Randomized Design, two factors which are 4 levels of 2,4-D concentrations: 0; 325; 400; 475 µM and 4 concentrations of BAP: 0; 25; 50; 75 µM. The result of statistic analysis indicated that the interaction of 2,4-D and BAP give have no significant effect of callus growth in culture and the percentage of callus fresh weight. The result showed that the treatment 2,4-D 475 µM without BAP can result the fastest time of callus induction. Meanwhile, the best treatment of callus fresh weight found in 2,4-D 400 µM and BAP 25 µM. Percentage of the culture that formed embriogenic callus is 44,44% and produced yellow callus 55,56%, yellow brown callus 30,56% and brown callus 13,88%.