Potensi Bakteri Asam Laktat yang Diisolasi dari Nira Aren dalam Menghambat Pertumbuhan Bakteri Patogen Asal Pangan

Abstract

Nira contain sugar and other nutritional components that are good for the growth of microorganisms, one of which lactic acid bacteria. The purpose of this study was to obtain lactic acid bacteria isolates potential of palm juice that could inhibit the growth of pathogenic bacteria of food origin and included in the candidate probiotics. Fermentation at various time intervals (0, 12, 24, 36 and 48 hours) palm juice, obtained LAB isolates were grown on media de mann rogosa agar (MRSA) + 1% CaCO3. Each of LAB isolates tested for their ability to inhibit pathogenic bacteria origin of food (Salmonella typhi, Staphylococcus aureus and Escherichia coli) on media Mueller Hinton Agar (MHA) with disc diffusion method. Isolates potential was inhibited pathogenic bacteria origin of food tested for its ability to survive in the atmosphere of the gastrointestinal tract such as gastric pH (2.0), intestinal pH (7.2), bile salts (0.5% ox gall) and capable of forming a biofilm on the surface of solid stainless steel on media MRSA ,origin isolated palm juice obtained 16 isolates of Lactic acid bacteria are grown on media MRSA CaCO3 + 1% and is dominated by heterofermentatif bacteria. There were only 6 isolates (MF5, MF10, MF11, MF13, MF14 and MF15) capable potentially inhibited the growth of pathogenic bacteria of food origin and isolates were also able to produce antimicrobial compounds other than organic acids. Only 5 out of 6 isolates that were able to survive in the atmosphere of the gastrointestinal tract and is able to form a biofilm on the surface of solid stainless steel namely MF5, MF10, MF11, MF14 and MF15, while isolates MF13 are not able to survive in an atmosphere of bile salts that are removed from the isolates candidates probiotics From all of isolates, there were two the most potentially isolates as a probiotic candidate namely MF14 and MF11.