Isolasi dan Uji Ekstrak Metanol Bakteri Endofit Keji Beling (Strobilanthes crispus) dalam Menghambat Pertumbuhan Beberapa Mikroba Patogen

Abstract

A study on isolation and examination methanol extract of endophytic bacteria from keji beling (Strobilanthes crispus) in inhibiting Salmonella typhii, Streptococcus mutans, Escherichia coli and Aspergillus flavus microbe growth was conducted. The objective of the study was to isolate endophytic bacteria producing antimicrobial compounds. There were 12 isolates of endophytic bacteria isolated from “keji beling”. Two isolates, AJ5 and AJ7 were chosen to be extracted with methanol based on their ability to inhibit the pathogen growth. The ability of the extract to inhibit the gowth of the pathogens were conducted at concentration of 40%, 60%, 80%, and 100%. The results showed that higher inhibition zone against Salmonella typhii was shown by the extract of AJ7 at concentration of 60% by 14 mm, Streptococcus mutans was shown by extract of AJ7 at concentration of 40% with inhibition zone of 8.4 mm, respectively, while extract of AJ5 with concentration of 40% and 60% inhibited Escherichia coli by 10 mm. Inhibition of fungi Aspergillus flavus was shown by the extract of AJ7 at concentration of 100% by 6.6 mm.