Produksi Katekin pada Kultur in Vitro Kalus Teh (Camellia sinensis L.) dengan Elisitasi Saccharomyces cerevisiae

Abstract

The study of “Catechin Production of In Vitro Culture of Tea Callus (Camellia sinensis L.) was elicitated by Saccharomyces cerevisiae” has been done from March 2014 to October 2014 at Plant Tissue Culture Laboratory of Mathematics and Science Faculty of Sumatera Utara University, and Trial Quality and Pesticides Residues Laboratory of The Technical Excutive Unit a Protect Crops and Horticulture in Medan. The aim of this research was to know the concentration of Saccharomyces cerevisiae’s cell walls elicitors to increase cathechin production. The experimental design was completely randomized non factorial using Murashige and Skoog medium with 1 mg/L of 2,4-D and 1 mg/L of kinetin that was added with Saccharomyces cerevisiae’s cell walls elicitors (with numbers of elicitor’s concentration level). The statistical analysis showed that in Murashige and Skoog medium with 1 mg/L of 2,4-D and 1 mg/L of kinetin as control medium gave the best effect to increase fresh weights of tea callus (Camellia sinensis L.), the increase of Saccharomyces cerevisiae’s cell walls elicitors tend to cause an increase in the dry weights of tea callus. 0,50% is the best elicitor’s concentration that is able to increase highest catechin production of 2,105 mg/g DW or increases 4 times compared to control.