Isolasi dan Potensi Bakteri Keratinolitik dari Feses Ular Sanca (Python sp.) dalam Mendegradasi Limbah Keratin

Abstract

Snake like Python sp. can eat bird including its whole feather and excrete feces with no feather left. It has been assumed that there are keratinolytic bacteria in the intestine of snake. In this study an isolation and an assay of potential of keratinolytic bacteria from feces of Python sp. has been conducted. A total of 10 bacteria were isolated and tested for their abilities to produce keratinase on feather meal agar. Two isolates, FU7 and FU10, were selected based on keratinolytic index for further study. Their abilities to degrade keratin were assayed in liquid medium containing chicken feather and goat hair as carbon and nitrogen source for 25 days of incubation. The result showed that FU7 degraded chicken feather and goat hair by 62,5% and 35,9%, respectively, while FU10 degraded both substrates by 56,9% and 19,2%, respectively.