Isolasi dan Uji Ekstrak Bakteri Asam Laktat Perairan Tawar dalam Mengendalikan Biofilm Aeromonas salmonicida pada Berbagai Permukaan Padat

Abstract

Aeromonassalmonicida is a bacterium that causefurunculosis on fish and is able to form biofilm on solid surface. The aim of this research is to control biofilm Aeromonassalmonicida using antimicrobial compound of Lactid Acid Bacteria (LAB) isolated from fresh water of intestinum gold fish (Cyprinuscarpio) pond around Medan city, North Sumatera. There were 12 (LAB isolated) from fresh water. The selected isolate, UM1, was found as a potential biocontrol agent of Aeromonassalmonicida which showed the highest inhibition 8.5 mmcompared to other 12 isolates. Further more UM1 was used to produce antimicrobial compound to control biofilm cell’s of Aeromonassalmonicida on gold fish scale and polyvinyl chloride (PVC). Biofilm formation of Aeromonassalmonicidawas performed in 1, 3 and 5 days in Nutrient Broth on fish scale and PVC then were detached with micro glass bead for bacterial counting. The highestnumber of biofilm cells were found at 3 days of incubation, 0.74 x 109 CFU/unit on PVC and 0.90 x 109 CFU/unit on fish scale. Controling of biofilm cell of Aeromonassalmonicida with antimicrobial compound of LAB isolate reduced up to 101 CFU/unit biofilm cell of Aeromonassalmonicida.