Karakterisasi Cangkang Kepiting Laut dan Kitin Serta Karakterisasi Kitosan dari Hasil Deasetilasi.

Abstract

Chitosan is the result of deacetylation of chitin produced from crab shells sea isolation. Chitin extraction stage includes the demineralization with 1 M HCl aimed at the removal of minerals contained in crab shells sea and deproteinasi with NaOH 3.5%, which aims to eliminate the protein. The transformation of chitin into chitosan is done by means of deacetylation with NaOH 50% aiming to decide an acetyl group. From the results of research conducted in the characterization obtained as follows: the yield of the transformation of the shell kepingting to demineralization phase was 25,4695 %, of demineralization stages toward chitin is 61.1116%, and from chitin into chitosan is 90,84 %, water content which obtained 6.76%, solubility chitin and chitosan in acetic acid 10% by respectively 12.3% and 0.17%, floured texture, degree of deacetylation chitosan with FTIR method is 99 .006%. Mineral analysis using AAS with a comparison between the shells of crab and results of demineralization by sequence on Ca (5.22%: <0,001 ppm), Mg (1.01%: 0.003%), Na (15.98%: 0.08% ), Zn (14.54 ppm: 9.28 ppm), Cu (3.04 ppm: 2.42 ppm), Fe (256.55 ppm: 38.78 ppm). Volume protein analysis method by comparison shells of crab, the results of demineralization and with chitin from deproteinasi results are respectively 13.08%, 43.33% and 39.61%.