Pengaruh Penambahan Ammonium Sulfat (NH4)2SO4 dan Waktu Perendaman Buffer Fosfat terhadap Perolehan Crude Papain dari Daun Pepaya (Carica papaya, L.)

Abstract

Papaya leaves is a plant that rich in benefits. Papaya leaves contains papain enzyme which is a protease enzyme that very helpful for the industry. This study aims to determine the effect of the saturation level of ammonium sulfate (NH4)2SO4 and immersion time with phosphate buffer to get yield of protease from crude papaya. In this study varied immersion time with phosphate buffer is 0, 12, 24, and 36 hours and the saturation level of ammonium sulfate (NH4)2SO4 is 40, 50, 60, 70, 80, and 90 %. The results of the analysis performed using UV spectrophotometer to determine yield, and water content. The best research results obtained immersion time with phosphate buffer at 36 h, with a saturation level of ammonium sulfate (NH4)2SO4 at 60 % is 132,9778 unit/ml.