“Pengaruh Pemberian Ekstrak Buah Lada Hitam (Piper nigrum L.) terhadap Pigmen Melanin Kulit Mencit (Mus Musculus L.) Hitam Model Vitiligo

Abstract

Black pepper fruit contains piperine compounds which may be responsible for producing skin pigmentation. This study aims to determine the effect of using blackpepper fruit extract on the extent of vitiligo, percent repigmentation and the amount of melanin pigment. This study used 25 black mice (Mus musculus L.) as test animals. Black mice used monobenzone to make animal models of vitiligo. This study used a completely randomized design method (CRD) consisting of 5 treatment groups, namely K- (administration of monobenzone), K+ (administration of monobenzone and commercial ointment), P1 (administration of monobenzone and black-pepper fruit extract 25%), P2 (administration of monobenzone and black-pepper fruit extract 50%), and P3 (administration of monobenzone and black-pepper fruit extract 75%). Observations in this study were carried out visually and histologically. The results showed that the administration of black-pepper fruit extract with a concentration of 75% was the best in reducing the area of vitiligo, increasing the percentage of repigmentation and the amount of melanin pigment with a vitiligo area of 0.118 cm2, the percentage of repigmentation of 75.34% and the amount of melanin pigment as much as 512.16, melanin pigment all of which were characterized by the presence of pigmentation spots.