Deteksi Virus Chikungunya pada Nyamuk Aedes aegypti dengan Menggunakan Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) di Empat Desa di Kabupaten Serdang Bedagai

Abstract

Background: In Indonesi4 chikungunya infection has been found since eighteenth century. At that time chikungunya infection was well known as joint fever. In Indonesia chikungunya outbreak was reported on 1973 at Samarinda and DKI Jakarta. From 2000-2007 in Indonesia, chikungunya outbreak occurred in all provinces with 18.169 cases without mortality. Now, almost all of the provinces in Indonesia we potential for chikungunya outbreak. Chikungunya is a disease caused by chikungunya virus, which is hansmitted by mosquitoes Ae. aegtpti and Ae. albopictus. Many breeding places of mosquitoes is related to increasing of chikungunya infection rate. Methods: This was a descriptive study for 50 samples of Ae. aegtpti by using Reverse-Transcriptase Polymerase Chain Reaction technique. Mosquitoes were caught from four villages of two endemis chikungunya subdishicts that is Sei Rampah subdistrict and Sei Silinda subdistric. Each of the villages, as much as ten mosquitoes were caught on morning to afternoon. Result: The result showed 13 samples were positive for chikungunya virus from 50 samples which were examined. Minimum Infection Rate is l30o/@. I)iscussion: Chikungunya viral n Ae. aegtpti was detected by RT-PCR technique in four villages in Sergai Regency