Organogenesis dari Kelapa Sawit Asal Eksplan Tunas Pucuk (Apical Bud)

Abstract

The purpose of this research is to find out explants apical bud from oil palm of different position which are apex, middle, and basal segment, were cultured in Y3 medium suplemented with 2,4-D in the presence of activated charcoal as many 3 g/L for induction organogenesis from callus inisiation. This research is conducted at Tissue Culture Laboratory Natural Sains University North Sumatera from November 2013 to June 2014. In this research, experiment for organogenesis by growth regulator substance 2,4-D at concentration of 100 mg/L, 115 mg/L, 120 mg/L, 135 mg/L, and 140 mg/L. This research was designed by completely randomized design two factorials with 5 attempt. The production of organ was affected by 2,4-D concentration and explants position; 2,4-D 100 mg/L was the most effective (66%) in inducing organogenesis from the basal segment.