Gambaran Histologis Hepar Mencit (Mus Musculus L.) Model Diabetes Tipe-2 Setelah Pemberian Ekstrak Etanol Daun Afrika (Vernonia Amygdalina Del.)

Abstract

An experiment of the histological profile of the mice’s liver (Mus musculus L.) suffered of type-2 diabetes model and applied by African leaf extract (Vernonia amygdalina Del.) was carried out from May to August 2022. This study aims to determine the safety by African leaf extract on the liver by looking at the hepatic organ weight, morphological and histological damage of the liver of mice induced by streptozotocin. This study used a completely randomized design (CRD) with 6 treatment groups, which were negative control (treatment group), diabetes induced by streptozotocin at a dose of 40 mg/kg BW (positive control), mice induced by diabetes African leaf extract at a dose of 100, 200, 300. mg/kg BW, and the metformin group at a dose of 65 mg/kg BW. The treatments were carried out for 30 days and then, the livers were taken to measure the weight and observed the morphology of the liver. The histological liver profile prepared by using the paraffin method with Hematoxylin-Eosin (HE) staining. The African leaf extract treatment group at a dose of 100-200 mg/kg BW reduce liver weight into the normal range, by an average value of 1.5 grams. The treatment at a dose of 200 mg/kg BW reduced the morphological and the histological damage of the liver structures with an average score of 2.24, and it also reduced the level of liver fibrosis damage in streptozotocin-induced mice with an average value of 2.33. The results showed that the effect of African leaf extract was safe for the liver. The group giving African leaf extract at a dose of 100-200 mg/kg BW.