| dc.description.abstract | Isolation phenolic compounds (Methyl gallate and 4’,6,7,8 – tetrahydroxy, 5 –
methoxy flavanone) from the stem bark of the Mara plant (Macaranga tanarius (L.)
Mull. Arg) were isolated. 2300 g of Mara plant stem bark were macerated by
methanol, and the methanol extract dissolved in water. The water solution was
extracted by partitioning with ethyl acetate and then concentrated. The ethyl acetate
extract was dissolved in methanol and extracted by partitioning with n-hexane until
the n-hexane layer was transparent, yielding 18.8 g of total flavonoids. Total
flavonoids from Mara plant stem bark were separated by column chromatography with
silica gel as the stationary phase and chloroform: methanol (90:10, 80:20, 70:30, and
60:40) v/v as the mobile phase. Fraction F1 + F2 was purified by preparative thin
layer chromatography using benzene : acetone (80:20) v/v to a produce armophous
solid of 8,2 mg and fractions F3 – F6 was purified by preparative thin layer
chromatography using chloroform : methanol (80:20) v/v to a produce armophous
solid of 4,4 mg. The resulting pure compounds were determined using UV-Visible, FTIR,
and 1H-NMR spectrophotometers. The Antibacterial activity of total flavonoid was
determined by agar disk diffusion against Staphylococcus aureus and Escherichia coli
bacteria. The results showed that total flavonoids from the stem bark of the Mara plant
(Macaranga tanarius (L.) Mull. ) strongly inhibit the growth of Staphylococcus aureus
and Escherichia coli bacteria with the MIC (Minimum Inhibitory Concentration) value
of total flavonoids from the stem bark of Mara plants (M. tanarius (L.) Mull. Arg) at a
concentration of 100 mg/mL obtained a clear zone of 11.1 mm against Staphylococcus
aureus bacteria and at a concentration of 100 mg/mL obtained a clear zone of 11.7
mm against Escherichia coli bacteria. | en_US |
