Efek Ekstrak Daun Afrika (Vernonia Amygdalina Del.) Terhadap Gambaran Histologi Ginjal Mencit (Mus Musculus L.) Model Diabetes

Abstract

Research on the effects of African leaf extract (Vernonia amygdalina Del.) on the histological appearance of the kidneys of mice (Mus musculus L.) the diabetes model has been carried out from May 2022 to July 2022. African leaves are a shrub that is used as an anti-diabetic. African leaves have the benefit of helping reduce kidney damage and can restore kidney function in diabetics. In this study, a completely randomized design (CRD) was used with 6 treatments and 4 replications. The treatment consisted of normal control, positive control, African leaf extract treatment at a dose of 100 mg/kg BW, 200 mg/kg BW, 300 mg/kg BW and metformin at a dose of 65 mg/kg BW. Kidney organ preparations were prepared using the parifine method and staining with phloxine hematoxylin. The results of inducing diabetes with Streptozotocin showed that there was a decrease in the P3 treatment (Streptozotocin 40 mg/kg body weight + African leaf extract 300 mg/kg body weight) namely in body weight, kidney weight, kidney length and kidney volume, glomerular basement membrane thickness and expansion of mesangial cells with an average of (body weight 29 grams), (kidney weight 0.625 grams), (kidney length 1.125 cm, kidney volume 0.225 mm3), (glomerular basement membrane thickness 0.46 mm3), (mesangial cell expansion 22,2). The results showed that African leaf extract was able to reduce body weight and kidney weight at doses of 100 mg/kg body weight and 200 mg/kg body weight and could reduce the thickness of the basement membrane and the expansion of kidney mesangial cells in diabetic mice.