Studi NCBI dan Marka Biokimia Klon Quick Starter dan Slow Starter Tanaman Karet (Hevea Brassiliensis Muell Arg.)

Abstract

Research on the rubber plant H. brasiliensis Muell Arg. has been carried out by various agencies and previous researchers, and the results of these researches have been published in various journals and are well documented in large numbers, but research that focuses on rubber biotechnology information is still limited. Researchers report applying a database search from the National Center for Biotechnology Information (NCBI) (GQuery) to gain insight into more valuable information about the latest biotechnology in the rubber plant H. brasiliensis. There are 26 databases found for H. brasiliensis. All rubber plant databases consist of literature, genes, genes, protein genomes, and chemical properties of H. brasiliensis. Furthermore, through the NCBI database, information about the polyprenol reductase gene was also collected. And with the same method, research on the acetyl CoA carboxylase gene was conducted. The Acetyl-CoA carboxylase gene was taken from six variants (NCBI): 1-like, X1, X2, X3, X4 and X5. To analyze a number of physico-chemical parameters, Four consistencies were observed: chloroplast transit peptide, mitochondrial target peptide, signal peptide of the secretory pathway, and prediction of their reliability. where the 1-like variant shows a difference from the others. The need for information about Acetyl CoA carboxylase is related to several studies, that ethylene in Hevea skin tissue regulates two main pathways to increase latex production in which acetyl CoA plays a role: a) increasing rubber synthesis, and b) prolonging latex flow. Research through extracting information from the National Center for Biotechnology Information (NCBI) database has provided information about rubber plants and confirmed the presence of the polyprenol reductase gene and the acetyl CoA carboxylase gene. The grouping of rubber clones based on latex metabolism is important as a reference for planters for the selection of planting material to be used on a commercial scale. In addition, it is also important to determine the expected composition of latex production in each field so that it can be sustainable. In this study, the grouping was done based on the composition of polyisoprenoid. More specifically, the polyisoprenoid composition in rubber tree leaves in Indonesia with the typology of quick starter and slow starter clones showed the presence of polyprenol and dolichol, with a slightly higher polyprenol content than dolichol. In H. brasiliensis leaves, polyprenol was detected, especially at IRR 5 as a quick starter and PB 217 as a slow starter clone. In this study, most of the clones (93.9%) were detected as type II in the presence of mutually polyprenol and dolichol with 31 clones of 33 entres clones. Two clones, IRR 5 and PB 217 were classified as type III, with the majority of polyprenols over dolichol. Furthermore, type I polyisoprenoids had dolichol dominance over polyprenols, which was not detected. The abundance and profile of polyisoprenoids in the rubber tree species Hevea brasiliensis with the typology of quick starter (QS) and slow starter (SS) clones in Indonesia was reported for the first time. And the dendrogram analysis did not show the relationship of entres clones, but showed similarity of clones focused on polyisoprenoid data on carbon chain lengths.