Isolasi dan Skrining Bakteri Selulolitik dari Usus Belalang (Oxya Chinensis) dan Potensi Selulolitiknya

Abstract

Grasshoppers are herbivorous insects or plant eaters which in large quantities can cause losses to farmers. Many studies have been conducted on cellulolytic microbes from herbivorous insects. This study aims to obtain bacterial isolates from the guts of grasshoppers (Oxya chinensis), to determine the diversity of cellulolytic bacteria and to determine their potential to produce cellulase enzymes. There are were obtained 27 isolates from 3 parts of the grasshopper intestine (foregut, midgut, hindgut). Cellulolytic bacteria are characterized by the formation of clear zones on CMC agar media after being flooded with 0.1% Congo red. All bacterial isolates have the ability to hydrolyze cellulose with a cellulolytic index ranges from 0,12 to 1,23. Based on the molecular identification of potential bacterial isolates indicated 5 different species, namely from the foregut section found Bacillus wiedmanniii (OCF2), midgut section found Bacillus marcorestinctum (OCM4), Bacillus halotolerans (OCM8) and hindgut section found Paenibacillus zanthoxyli (OCH4), Bacillus hominis (OCH7). Bacillus wiedmanniii (OCF2) had the highest cellulase enzyme activity of 0,5830 U/mL and the highest cellulase specific activity of 0,0068 U/mg.