| dc.description.abstract | Grasshoppers are herbivorous insects or plant eaters which in large
quantities can cause losses to farmers. Many studies have been conducted on
cellulolytic microbes from herbivorous insects. This study aims to obtain bacterial
isolates from the guts of grasshoppers (Oxya chinensis), to determine the diversity
of cellulolytic bacteria and to determine their potential to produce cellulase
enzymes. There are were obtained 27 isolates from 3 parts of the grasshopper
intestine (foregut, midgut, hindgut). Cellulolytic bacteria are characterized by the
formation of clear zones on CMC agar media after being flooded with 0.1% Congo
red. All bacterial isolates have the ability to hydrolyze cellulose with a cellulolytic
index ranges from 0,12 to 1,23. Based on the molecular identification of potential
bacterial isolates indicated 5 different species, namely from the foregut section
found Bacillus wiedmanniii (OCF2), midgut section found Bacillus
marcorestinctum (OCM4), Bacillus halotolerans (OCM8) and hindgut section
found Paenibacillus zanthoxyli (OCH4), Bacillus hominis (OCH7). Bacillus
wiedmanniii (OCF2) had the highest cellulase enzyme activity of 0,5830 U/mL and
the highest cellulase specific activity of 0,0068 U/mg. | en_US |
