Analisis Kariotipe Kromosom Ikan Balon Sailfin Molly (Poecilia latipinna Lesueur, 1821)

Abstract

Research on sailfin molly fish has never been done, so information on the karyotype, pattern, shape, and size of the chromosomes of sailfin molly has not been found. This study was conducted to determine the number of chromosomes and the diversity of fish chromosomes. Methods performed with the descriptive method. Samples were collected from fish vendors and brought to the Genetic and Biology Molecular Laboratory to be acclimeted for 7 days. Fishes were administered by Colchicine 0.007% for 12 hours to obtain a chromosome at the metaphase stage. Preparation of the cell was done by solid tissue technique using organs in the form of gills continued the process of coloring using Giemsa 5% and the observation of preparation on a microscope with the help of an amscope. Karyotype data analysis using Adobe Photoshop CS3 and Ideokar 1.3 software applications obtained chromosome pairs based on the shape and length of the chromosomes. In this study, the Sailfin molly balloon fish chromosome was found with a total of 42 chromosomes, or 21 pairs of metacentric-shaped chromosomes on 19 chromosomes and submetasentric on 2 chromosomes. Largest long arm chromosome 12.06; short-arm chromosome 9.3 with a total number of 21.36 in the ratio of chromosomal arms of 1.76 with a relative length percentage of 9.95 chromosomes and a percentage of chromosome shape of 1.76 and a centromere index of 0.49