| dc.description.abstract | Maculabatis sp. is the fisheries resources of the Malacca Strait waters in the
Endangered category. Monitoring is efforts to conserve this species. The
complexity is correctly identifying this species, causing limited information,
Consequently, genetic identification is required in order to gather reliable
information on this species. The objective of this research is to identify molecular
and phylogenetic analyses of star rays (Maculabatis sp.) landing at Tanjung Tiram
Fish Port. Primary data from whipray tissue samples, secondary data using the
National Center of Biotechnology Information (NCBI) database. Research Stages
is started with the preparation of the sample tissue that is preserved, then DNA
extraction, followed by amplification using the Polymerase Chain Reaction
method and then electrophoresis. The amplification results were sequenced,
aligned using MEGA X software with the Basic Local Alignment Tool.
Phylogenetic and genetic distance analysis using Kimura 2P, maximum likelihood
with 1000 bootstrap. Research results Sample 1 (TP1) is Maculabatis gerardi and
sample 2 (TP2) is Maculabatis gerardi. Based on the results of phylogenetic
analysis, samples A (TP1) and sample B (TP2) are incorporated in 1 clade and
have an accuracy rate of 100% in other clades. 4 subclades with the species
Maculabatis pastinacoides, Maculabatis astra, Maculabatis randalli, Himantura
toshi, Maculabatis macrura and Maculabatis gerardi and have bootstrapping
values varying 59%, 87%, 100% with the farthest Nucleotide P-distance at
GU673583 Maculabatis randalli whipray with a value of 0.209 and the closest is
MG774924.1 Maculabatis macrura whipray with a value of 0.131, indicating a
difference in genetic distance between species. | en_US |
