Analisis Metabolit Sekunder Melalui Kultur Suspensi Sel Kalus Jintan Hitam (Nigella sativa L.) dengan ZPT Picloram dan BAP pada Kombinasi Media MS

Abstract

Analysis of Secondary Metabolites Through Callus Cell Suspension Cultures of Black Cumin (Nigella sativa L.) With PGR Picloram and BAP in Combination MS Media, supervised by Revandy Iskandar Muda Damanik. The aim of the research was to determine the effect picloram and BAP and the combination of MS media on cell suspension cultures and secondary metabolites contained in callus of black cumin (Nigella sativa L.). This research was conducted at the Tissue Culture Laboratory, Faculty of Agriculture and Phytochemical Laboratory, Faculty of Pharmacy, University of North Sumatera from February 2023 to July 2023. The Completely Randomized Design (CRD) method was used with factors and each factor with 3 replications. The first factor was plant growth regulator concentration in MS medium (Z): Z1 (Picloram 0 ppm + BAP 1 ppm), Z2 (Picloram 1 ppm + BAP 1 ppm), Z3 (Picloram 1 ppm + BAP 2 ppm), Z4 (Picloram 2 ppm + BAP 2 ppm). The second factor was the composition of MS media (M): M1 (½ MS) and M2 (MS). Parameters observed were callus morphology, growth index, SCV (Settled Cell Volume), PCV (Peacked Cell Volume) and analysis of secondary metabolite content. The results showed that the combination of MS medium and concentrations of picloram and BAP had no significant effect on all parameters. Z4 and M2 concentrations provided the most optimal growth in this study.