| dc.description.abstract | Effect of 2,4-Dichlorophenoxyacetate (2,4-
D) Application at Several Media Concentrations on Black Orchid (Coelogyne
pandurata) In Vitro Culture, supervised by Emmy Harso Kardhinata. This study
aims to determine the effect of MS media concentration and 2,4-D and their
interaction on the growth of black orchid in vitro. This research was conducted at
the Tissue Culture Laboratory, Alifa Agricultural Research Center (ALIFA-ARC),
from April to June 2023. This study used a Completely Randomized Design (CRD)
method with 2 treatment factors. The first factor is the concentration of MS
Media: half MS and full MS. The second factor is the concentration of 2,4 D: (0
mg/L, 2 mg/L, 4 mg/L, 6 mg/L and 8 mg/L). There were 10 treatment
combinations with 3 replications resulting in 50 experimental units. The
parameters measured were: percentage of living explants (%), percentage of
contaminated explants (%), number of shoots per explant (fruit), shoot height
(cm), number of roots (fruit) and root length (cm). The results showed that the
treatment of MS media concentration had no significant effect on black orchid
tissue culture on all observed parameters. The treatment of 2,4 D concentration
had no significant effect on black orchid tissue culture on all observed
parameters. The interaction between MS media concentration and 2,4 D had no
significant effect on black orchid tissue culture on all observed parameters. In
addition, it can be seen that M0D1 treatment is the best combination for all
observed parameters. | en_US |
