Isolasi dan Identifikasi Mikroskopis Jamur dari Kompleks Jaringan Terinfeksi Penyakit Layu pada Tanaman Bayam Jepang (Spinacia oleracea L.) dan Uji Patogenesitas Secara Invivo

Abstract

This research was aimed to detect pathogenic fungi from tissue complexes of wilt disease on Japanese Spinach plants(Spinacia oleracea L.) and invivo pathogenicity test. The research was conducted at the Plant Disease Laboratory of the Agrotechnology Study Program, Faculty of Agriculture, Universitas Sumatera Utara, Medan and in the agricultural land in Sempajaya Village Kec. Berastagi Regency Karo from December 2020 to October2022. This research used a completely randomized design (CRD) non factorial 5 treatment which came from infected Japanese spinach plant tissue, with the following treatments (F1,F2,F3,F4 and F5) each reolicate consisted of 3 experimental units. The results of the research showed that the isolation and microscopic identification of 4 Fusarium isolates were found, each isolate differed microscopically in several characteristics of macroconidia, microconidia and chlamydiospores. Invivo pathogenicity test of 4 Fusarium isolates, respectively Fusarium spp.,1, Fusarium spp.,2, Fusarium spp.,3, dan Fusarium spp.,4. Shows that isolates of Fusarium spp.,1, and Fusarium spp.,2 are pathogenic to Japanese spinach plants, causing disease severity up to 100 %.