Induksi Kalus, Protocorm-Like Bodies (PLBs), Dan Tunas Dendrobium sp. pada Media MS dengan Variasi Kombinasi Benzyl Amino Purine (BAP) dan Auksin

Abstract

Conventional propagation of Dendrobium orchids requires a relatively longer time and low success rate, so the propagation technique needs to be carried out using tissue culture techniques, among which by varying growth regulators (PGRs). This study aims to obtain the optimal combination of growth regulators (PGRs) to induce the formation of callus, buds, and protocorm-like bodies (PLBs) in Dendrobium orchids. The research design used a Completely Randomized Design (CRD) consisting of 10 treatments in the form of a combination of BAP (2 mL/L) and 3 types of auxins namely 2,4-D, NAA, IAA. The concentration of each type of auxin used was 2, 4, and 6 mL/L. Control was the medium without the addition of BAP and auxin. The research material was stem explants of Dendrobium planlets. The results showed that the combination of BAP and 3 types of auxin had a significant effect on the induction of callus, shoots and PLBs. Explants grown on medium without the addition of PGRs (control) can induce callus faster than the medium treated with PGRs, with an average growth of 10.33 days. The highest number of PLBs was also produced in the control treatment with a total of 2.67 PLBs while the average growth of PLBs, initiation of shoot growth and the best number of shoots in the medium with a combination of BAP (2 ml/L) and IAA (2 ml/L).