Pengaruh Kitosan Terhadap Pertumbuhan Protocorm-Like Bodies Dendrobium Sp.

Abstract

Plant tissue culture is one of several techniques use to multiply orchids. Using plant growth regulators on plant tissue culture can accelerate plant growth. Chitosan can be used as a growth promoter of orchids. This study aims to determine the effect of chitosan on the growth of protocorm-like bodies of Dendrobium sp. The research was conducted from February to May 2023. The materials used in this research was protocorm-like bodies of Dendrobium sp. grown in MS (Murashige and Skoog) medium adding 20% coconut water and several concentrations of chitosan. This study was designed in a non-factorial completely randomized design with five replication on five treatments.The treatments consisted of control, MS Media + 20% Coconut water + Chitosan 2.5 mg/L, MS Media + 20% Coconut water + Chitosan 5 mg/L, MS Media + Coconut water 20% + Chitosan 10 mg/L, and MS Media + Coconut water 20% + Chitosan 15 mg/L. The results showed that the treatment with Media MS + Coconut water 20% + Chitosan 15 mg/L produced the highest average of the shoots, the leaves, the lamina area, the roots, the protocorm-like bodies, the plantlets height and the fresh weight. While average of the highest root length, showed on control and treatment MS Media + Coconut water 20% + Chitosan 10 mg/L.