| dc.description.abstract | Isolation and ident fication of flavonoid compounds from henna fruit (Lawsonia inermis L.) through phytochemical screening where the ethyl acetate extract of henna fruit is positiveforflavonoids with 5% FeCl3 reagent and Mg (s) + HCl (p), then extracted maceration with methanol solvent, then re-extracted with ethyl acetate, partitioned with n-hexane and obtained methanol concentrated extract. The methanol concentrated extract was separated by column chromatography with silica gel stationa1y phase and chloroform: ethyl acetate mobile phase. Fractions 150-185 were pur fied by preparative thin layer chromatography. The pure compound obtained is a yellow amorphous solid, has a mass of 12 mg and an Rf price of 0.72 with chloroform: ethyl acetate eluent 30:70 (vl v) and an Rf value of 0.78 with acetone: benzene eluent 40:60 (v/v). UV-Vis spectroscopic analysis with methanol solvent showed two absorptions with wavelengths (J..max) 348 and 267 nm. FT-IR spectroscopy showed the presence of OH, C-H sp3, C=O ketone, C=C aromatic, O-H bending, C-0 bending, and C-0 -C asymmetric and symmetric bending groups at wave numbers 3425.58; 2958.80; 2873.94; 1728.22; 1512.19; 1379.10; 1125.57; 1072.42;
1037.70 (cm-1), respectively. Proton core magnetic resonance spectroscopy (JH NMR) showed the presence o_fprotons H-8, H-6, H-2: H-5', H-6'. Spectroscopic data showed that the isolated compounds wereflavonol group. | en_US |
