Penapisan Jamur Agens Pengendali Potensial pada Rhizosfer Daerah Endemik Patogen Magnaporthe grisea Penyebab Penyakit Blas pada Tanaman Padi (Oryza sativa L.)

Abstract

Rice are one of the main food sources for humans which are widely consumed by the world community. Rice cultivation in practice encounters several main obstacles, namely the attack of plant-disturbing organisms which can cause a decrease in rice production. Blast disease is the most common disease found in several locations and can reduce rice production by 30%. The aim of this study was to determine the abundance of antagonistic fungi from the rhizosphere which are found in endemic areas of the pathogen M. grisea causes blast disease in rice plants and determines which rhizosphere fungi are non-pathogenic in endemic areas of the pathogen M. grisea which causes blast disease in rice plants. The research was conducted by mapping the locations historically endemic to the pathogen M. grisea. After that, soil samples were taken and screened using physiological water and growing and purifying fungal colonies on PDA media. Identification of fungi using Slide Culture media is used to see fungal spores growing on the media. Furthermore, the pathogenicity test, morphological identification and antagonist test were carried out as the observed variables in this study. The diversity of Aspergillus spp., Penicillium spp. and Mucor spp. in the screening. The fungi found were not pathogenic to the surface-sterilized potato slices test medium. The highest inhibition was found in treatment B2 with a percentage of inhibition of 74.2% and the lowest with a percentage of inhibition of 8.7% in treatment B13. The secondary metabolites in the B2 treatment were thought to be the reason for the inhibition of the growth of the M. grisea pathogen in vitro.