Flavonoid Ekstrak Etanol Daun Bosi-Bosi (Timonius flavescens (Jacg) Baker) Metode Spektrofotometri UV-Vis

Abstract

Background : Bosi-bosi (Timonius flavescens (jacg.) Baker) has secondary metabolite compounds in the form of flavonoids, phenols, terpenoids, saponins, and vitamin C. Secondary metabolite compounds in bosi-bosi leaves are bioactive compounds that can be used as medicinal ingredients. Phenolic compounds are the largest secondary metabolite compounds in plants and are also natural ingredients whose use is quite widespread. Flavonoids are the main compounds derived from phenols that are easily found in natural ingredients. To determine the levels of total phenols and total flavonoids quantitatively, you can use the UV-Vis spectrophotometry method. Because phenolic and flavonoid compounds have chromophore groups and can produce absorption in the visible light area, their levels can be measured using a UVVis spectrophotometer instrument. Purpose : The aim of this research is to determine the levels of total phenols and total flavonoids in the ethanol extract of bosi-bosi (Timonius flavescens (jacg.) Baker) leaves using UV-Vis spectrophotometry. Method : Determination of total phenol and total flavonoid levels was carried out using the Folin-Ciocalteau preaction and the AlCl3 preaction on the ethanol extract of bosi-bosi leaves. Result : The yield of simplsia was 10% and the yield of extract was 10%. The total phenol content obtained in the ethanol extract of bosi-bosi leaves was 281.5384 mgGAE/g extract. The total flavonoid content obtained in the ethanol extract of bosi-bosi leaves was 21.1650 mgQE/g extract. Conclusion : Based on the research results, the total phenol content obtained in the ethanol extract of bosi-bosi leaves was 281.5384 mgGAE/g extract. The total flavonoid content obtained in the ethanol extract of bosi-bosi leaves was 21.1650 mgQE/g extract.