Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Biji Palem Palas Payung (Licuala Grandis (T.Moore) H.Wedl.)

Abstract

Palas payung palm (Licuala grandis (T.Moore) H.Wedl.) is a plant of the palm family (Arecaceae) that has long been used by the community to treat diarrhea, worms, boils, and as a natural dye. Isolation and identification of flavonoid compound from the palas payung palm seeds (Licuala grandis (T.Moore) H.Wedl.) had been carried out through the maceration extraction stage with methanol solvent. The methanol extract was concentrated then extracted with ethyl acetate and evaporated. The concentrated extract of ethyl acetate was dissolved with methanol and partition extracted with n-hexane. The methanol layer is then evaporated until all the methanol has evaporated. The concentrated methanol extract was then analyzed by TLC, then separated by column chromatography with silica gel as the stationary phase with n-hexane: ethyl acetate (90:10; 80:20; 70:30; 60:40; 50:50; 40:60; 30:70; 20:80; 10:90) v /v as mobile phase. The pure compound obtained was in the form of amorphous solid, mass = 6 mg with a value of Rf = 0.77 with an eluent of n-hexane: ethyl acetate (10:90) v /v .Based on UV-Vis spectroscopy analysis with methanol solvent, it shows that there are two absorptions with wavelengths (λ max) of 305 and 268 nm. The FT-IR spectroscopy shows the presence of OH groups, C-H sp3 stretch, C=O ketone, C=C aromatic, C-H bend, C-O-C stretch and C-H sp2 bend. Proton Nuclear Magnetic Resonance spectroscopy (1H-NMR) shows the presence of H-3, H-2’, H-5’, H-6’, and OCH3 protons. Based on data analysis that the isolated compound is a flavonoida compound of the flavon group.