Uji Pertumbuhan Bakteri Selulotik Simbion Larva Oryctes Rhinoceros pada Beberapa Media Pertumbuhan

Abstract

Oryctes rhinoceros larvae are one of the sources of cellulose and hemicellulose degrading bacteria. The growth of cellulotic bacteria is highly dependent on nutrient sources, energy sources and environmental conditions. Nutrient Agar (NA) is one of the most commonly used media for bacterial growth. The use of NA for bacterial growth media requires large costs and is considered to have no economic value. The use of alternative media for growth media of O. rhinoceros symbiont cellulotic bacteria that are cheap, easily available and contain sufficient nutrients for bacterial growth is needed. Soybean, tuna, and jackfruit seeds have high protein and carbohydrate content that can be utilised as a source of nitrogen and carbon for the growth of cellulolytic bacteria. The purpose of this study was to see the growth of cellulotic bacteria symbionts of O. rhinoceros on several growth media. The research was conducted at the Plant Disease Laboratory, Faculty of Agriculture and at the Microbiology Laboratory, Faculty of Mathematics and Natural Sciences, University of North Sumatra from January to May 2024. The research was conducted with experimental methods using a Completely Randomised Factorial Design with 2 factors and 3 replicates. The results showed that the use of alternative media can grow cellulolytic bacteria well. The best treatment was found in tuna flour media with an average number of colonies of 100.5 x10-6 cfu/ml.