Khamir Endofit Buah Sirsak (Annona Muricata Linn.) dan Kemampuannya Menghasilkan Asam Organik Penghambat Aspergillus Flavus dan Aflatoksin yang Dihasilkannya

Abstract

Soursop is a habitat for several species of endophytic yeasts that potential to produce organic acids. The aim of this study was to study endophytic yeasts of soursop i.e. Rhodotorula mucilaginosa, Rhodotorula sp., Kloeckera sp., and Candida sp. that produce organic acids that inhibit toxigenic Aspergillus flavus and aflatoxin production. The ability of yeasts in producting organic acid was conducted by in potato extract medium for 10 days (29℃). Toxigenicity A. flavus was determined using Coconut Agar Medium (CAM). The ability of each endophytic yeasts in producing organic acid was conducted by inoculating one ose of A. flavus at the center of the CAM medium in petri dishes. All plates were incubated for 5 days (29℃). The parameter determined based on colony diameter and the presence of yellow pigment on the bottom of the medium at petri dish. Result showed that all endophytic yeasts were potential to produce organic acid, inhibit A. flavus growth and aflatoxin production. Among endophytic yeasts, Candida sp. was the highest organic acid producer (0,00354%) followed by Rhodotorula sp. (0,00353%), whereas, Rhodotorula mucilaginosa was the highest growth inhibitor and aflatoxin production of A. flavus.