| dc.description.abstract | Andaliman (Zanthoxylum acanthopodium DC.) is a typical plant found in the
North Sumatra region. andaliman's ability to produce secondary metabolite
compounds can be used as a natural medicinal ingredient. One important part of the
andaliman plant that can produce many secondary metabolites is the leaves.
However, the lack of exploration of the potential of andaliman leaves as
antimicrobial producers, it is necessary to conduct research in determining the
antimicrobial activity of andaliman leaf extract against clinical pathogenic microbes.
The andaliman leaves were obtained from Pollung area, Humbang Hasundutan
Regency. The andaliman leaves were macerated with three types of solvents namely
ethanol, ethyl acetate, and n-hexane. Extracts from each solvent were made in
several concentrations, namely 15%, 25%, 35%, and 45%. The andaliman leaf
extract was tested for antimicrobial activity against pathogenic microbes i.e.
Escherichia coli, Staphylococcus aureus and Candida albicans with the kirby bauer
method. The results showed that the phytochemical screening of ethanol, ethyl
acetate, and n-hexane extracts of andaliman leaves contained secondary metabolite
compounds i.e. alkaloids, saponins, glycosides, steroids and triterpenoids, while
flavonoid and tannin compounds were only produced from ethanol extract of
andaliman leaves. The diameter of the inhibition zone of the three solvents against
pathogenic microbes indicates antimicrobial activity. The highest ability to inhibit
microbes was produced from ethanol extract with a concentration of 45%. The
concentration of the extract is directly proportional to the inhibition zone, namely the
higher the concentration of the extract, the greater the inhibition zone produced.
Antimicrobial tests show that bacteria that are more sensitive to andaliman leaf
extract are Staphylococcus aureus. This proves that andaliman leaf extract has the
potential to be developed as a natural antimicrobial in inhibiting clinical pathogenic
microbes. | en_US |
