Induksi Kalus Jintan Hitam (Nigella Sativa L.) dengan Menggunakan Kombinasi Zat Pengatur Tumbuh 2,4 Dichlorophenoxyacetic Acid (2,4-D) dan Kinetin pada Media MS

Abstract

Nigella sativa L. is a herbal plant commonly known to the public as black cumin seeds. The problem is that black cumin production in Indonesia is still small, so Indonesia imports black cumin seeds from India, Egypt, Syria and South Africa to meet the high demand for black cumin seeds. This study aims to determine the effect of using the growth regulator 2,4 D and the growth regulator kinetin as well as the interaction of the combination of 2,4 D and kinetin on the growth of black cumin (Nigella sativa L.) callus in vitro. This research used a Completely Randomized Design (CRD) method with 2 treatment factors and each treatment had 3 replications. The first treatment factor is the ZPT concentration of 2.4 D which consists of 0 ppm, 2 ppm and 4 ppm. The second treatment factor is the concentration of ZPT kinetin which consists of 0 ppm, 1 ppm and 2 ppm. The results of this study showed that administration of 2.4 D with a concentration of 2 ppm resulted in the best percentage of explants growing with callus and the fresh weight of the callus and 2.4 D with a concentration of 4 ppm showed the fastest callus emergence day. The administration of kinetin had a significant effect on observing the percentage of explant growth and the day black cumin callus appeared. Kinetin concentrations of 0 ppm and 2 ppm gave results that were not significantly different. The interaction between the administration of 2.4 D Growth Regulator and kinetin had a significant effect on observing the percentage of explant growth and the day callus appeared, but had no significant effect on observing the fresh weight of the callus.