Isolasi Fragmen Gen pup1 sebagai Gen Ketahanan Penyakit Blas pada Padi Siporang (Oryza Sativa L.) dari Sipirok, Sumatera Utara

Abstract

The decline in rice productivity can be caused by disease attack to the paddy, one of them is blast disease, it is caused by the hemibiotropic fungus Pyricularia oryzae. In North Sumatra, rice that resistant to blast disease was found, one of which is Siporang rice from Sipirok. Resistance to blast disease is encoded by several R genes, one of which is the pup1 gene. The pup1 gene is located on chromosome 12 and encodes the expression of dirigent-like protein, fatty acidα-dioxygenase, and aspartic proteinase which play a role in increasing the mechanism of plant resistance toward disease attack. This study aims to isolating the pup1 gene fragment and sequencing it to determine the sequence of bases that make up the pup1 gene nucleotide as a source of gene information for plant breeders. The research stages were carried out by isolating total rice DNA with the CTAB method, DNA amplification using pup1-specific primers, and sequencing analysis through the BLASTn and NCBI programs. The results showed that the isolated pup1 sequence has a 650 bp in size, and the results of BLASTn analysis showed high homology between the pup1 gene sequence of Siporang rice (Oryza sativa L.) to the pup1 gene sequence in Nipponbare rice cultivar (AL713911.3) with an identity of 96% and an e-value of 0.0 those indicates that the gene sequence analyzed is the correct pup1 gene sequence. Kinship analysis of the Siporang rice pup1 gene sequence with several rice sequences in GenBank clustered at a high level of similarity of 99.987%.