| dc.description.abstract | The decline in rice productivity can be caused by disease attack to the paddy, one of
them is blast disease, it is caused by the hemibiotropic fungus Pyricularia oryzae. In
North Sumatra, rice that resistant to blast disease was found, one of which is Siporang
rice from Sipirok. Resistance to blast disease is encoded by several R genes, one of
which is the pup1 gene. The pup1 gene is located on chromosome 12 and encodes the
expression of dirigent-like protein, fatty acidα-dioxygenase, and aspartic proteinase
which play a role in increasing the mechanism of plant resistance toward disease
attack. This study aims to isolating the pup1 gene fragment and sequencing it to
determine the sequence of bases that make up the pup1 gene nucleotide as a source of
gene information for plant breeders. The research stages were carried out by isolating
total rice DNA with the CTAB method, DNA amplification using pup1-specific
primers, and sequencing analysis through the BLASTn and NCBI programs. The
results showed that the isolated pup1 sequence has a 650 bp in size, and the results of
BLASTn analysis showed high homology between the pup1 gene sequence of Siporang
rice (Oryza sativa L.) to the pup1 gene sequence in Nipponbare rice cultivar
(AL713911.3) with an identity of 96% and an e-value of 0.0 those indicates that the
gene sequence analyzed is the correct pup1 gene sequence. Kinship analysis of the
Siporang rice pup1 gene sequence with several rice sequences in GenBank clustered
at a high level of similarity of 99.987%. | en_US |
