Isolasi dan Identifikasi Golongan Senyawa Fenolik dari Daun Sigar Jalak (Flueggea virosa (Roxb. Ex Willd.) Royle)

Abstract

The phenolic compound had been isolated from the leaves of sigar Jalak (Flueggea virosa (Roxb. Ex Willd.) Royle. Isolation was carried out by maceration extraction method with methanol and methanol extract was re extracted with ethyl acetate repeatedly until negative to 5% FeCl3. Ethyl acetate extract was concentrated and reconstituted with methanol and partitioned with n hexane untul n hexane layer was clear. The methanol extract was analyzed by thin layer chromatography and separated by coulumn chromatography with a stationary phase silica gel and a mobile phase of chloroform : methanol (90:10; 80:20; 70:30; 60:40) v/v. Fractions 41 121 was purified by preparatif thin layer chromatography using Chloroform : Methanol (80:20) v/v eluen to produce a greenish yellow amorphous solid of 8,1 mg at Rf value of 0,63 using Benzene : Aceton (80:20) v/v eluent. Based on the analysis of the UV Visible spectroscopy, it was wavelength (λmax) of 275 nm, the FT IR spectrum shows the presence of OH, C=C aromatic, C=O ester, C H, and C O groups. The proton Nucleus Magnetic Resonance Spectrum (1H NMR) shows the presence of H 2, H 6 and OCH3 protons. Based on the data analysis and interpretation, the isolated compound is a phenolic compound of the phenolic acid group which is suspected to be methyl gallate.