Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Kulit Batang Tumbuhan Jeruk Purut (Citrus hystryx D.C)

Abstract

Isolation and identification of flavonoid compounds from the bark of the kaffir lime plant (Citrus hystrix DC) begins with the maceration extraction stage with methanol solvent. The methanol extract was evaporated and concentrated using a rotary evaporator. The concentrated methanol extract was re-extracted with ethyl acetate, evaporated and concentrated using a rotary evaporator. The concentrated extract of ethyl acetate was dissolved with methanol and partition extracted with n-hexane. The methanol layer is evaporated until all the methanol has evaporated. The concentrated methanol extract was analyzed by TLC, then separated by column chromatography with silica gel as the stationary phase and chloroform: ethyl acetate (90:10, 80:20, 70: 30, 60:40) v/v as the mobile phase. The pure compound obtained was a yellow amorphous solid, mass = 4 mg with Rf = 0.55 using the eluent chloroform: ethyl acetate (90:10) v/v. Based on UV-Vis spectroscopy analysis with methanol solvent, it shows that there are two absorption peaks with wavelengths of 310 nm and 274 nm. The FT-IR spectrum shows the presence of O-H, C-H sp3, C=O ketone, C=C aromatic, C-H, C-O, and C-O-C groups and respectively at the wave number 3446.79; 2922.16; 1636.64; 1564.27; 1411.89; 1126.43; 1039.63 cm-1. Proton Nuclear Magnetic Resonance Spectrum (1H-NMR) shows the presence of H-3' and H-5', H-2' and H-6', H-2 and H-3 protons. Spectroscopic data shows that the isolated compound is a dihydroflavonol group.