Aktivitas Penyembuh Luka Sediaan Hidrogel Asap Cair dari Tempurung Kelapa (Cocos Nucifera L.) erhadap Ulkus Diabetikum pada Tikus Putih

Abstract

Background: Diabetic ulcer are chronic wounds in diabetes sufferers that are at risk of bacterial infection. Diabetic ulcer that are seriously infected with bacteria will turn into gangrene and must be amputated immediately. High levels of glucose in the blood can support bacterial growth. Coconut is a multipurpose plant, so the shell can also be used. Coconut shell liquid smoke is rich in phenol and acetic acid so it can be formulated into a hydrogel for wound healing. Objective: Evaluating the effectiveness of coconut shell liquid smoke hydrogel preparations in concentrations of 25%, 50%, and 75% as a diabetic wound healer using visual and histopathological wound healing parameters, the number of macrophages. Method: Liquid smoke was made using the pyrolysis and distillation method, then the liquid smoke hidrogel preparation which had been formulated according to the concentration, hydrogel base, and bioplacenton was observed for its wound healing effect on test animals that had been induced diabetes with nicotinamidestreptozotocin on day 3, day 3. -7, day 14, and day 21 visually and histopathologically. The research data was then tested statistically using SPSS 2022. Results: The 25% concentration coconut shell liquid smoke hydrogel preparation is the most effective preparation in healing diabetic wounds approaching bioplacenton on the market. Visually, it was found that the test animals recovered on the 14th day of observation with a wound length of 0 cm and a healing percentage of 100%. Histopathologically, the number of macrophages in mice decreased, namely 1.26: 2.46; 1.73 on consecutive treatment variations. Conclusion: Based on the research results, it can be concluded that the coconut shell liquid smoke hidrogel preparation can be used as an alternative therapy for healing diabetes wounds with the best wound healing effect shown by the hidrogel preparation with a concentration of 25%.