Biosintesis Nanopartikel Perak dari Ekstrak Daun Sukun (Artocarpus altilis) dan Aplikasinya sebagai Antioksidan dan Antibakteri

Abstract

Background: The preparation of nanoparticles that have a size below 100 nm is referred to as nanoparticle synthesis. Nowadays, biological approaches using green synthesis methods are becoming popular due to their environmental sustainability and simplicity. Objective: This study aims to synthesize silver nanoparticles using breadfruit leaf water extract, characterize silver nanoparticles, conduct antibacterial activity tests against Staphylococcus aureus and Staphylococcus epidermidis bacteria and Antioxidant Activity Testing of breadfruit leaf water extract and synthesized silver nanoparticles using the DPPH method. Methods: This research includes the preparation of breadfruit leaf water extract by heating for 30 minutes, synthesis of silver nanoparticles by green synthesis method using Hotplate method, characterization of silver nanoparticles which includes UV-Vis spectrophotometer, PSA, FTIR, SEM-EDS, antibacterial activity testing using dilution method with the help of Microplate, and Antioxidant Activity Testing using DPPH method. Results: The results of this study indicate that silver nanoparticles can be synthesized using water extract of breadfruit leaves. The resulting silver nanoparticles were characterized using UV-Vis Spectrophotometer which showed λ = 431 nm. Characterization using PSA showed a particle size of 68.43 nm. FTIR characterization of silver nanoparticles showed the presence of O-H bonds from phenol groups, C-H from ketone groups, C=O from aldehyde groups, and C-O from ester groups. SEM results show the agglomeration of some particles that have nano size. EDS results showed the presence of Ag (80.23%) which was dominant at the 3.0 keV peak. Silver nanoparticles showed antibacterial activity against Staphylococcus aureus with a minimum inhibitory concentration (KHM) at a concentration of 7.8 μg/mL and Staphylococcus epidermidis 15.6 μg/mL, with a minimum kill concentration (KBM) at Staphylococcus aureus 15.6 μg/mL and Staphylococcus epidermidis 31.2 μg/mL. The concentration of IC50 value on breadfruit leaf water extract is 157.36 ± 0.24 μg/mL indicating that the antioxidant activity is weak and on Silver Nanoparticles is 428.19 ± 0.18 μg/mL indicating that the antioxidant activity is weak. Conclusion: The results showed that silver nanoparticles can be synthesized using breadfruit leaf water extract bioreductors, have antibacterial activity against Staphylococcus aureus and Staphylococcus epidermidis and have weak antioxidant activity in breadfruit leaf water extract and synthesized silver nanoparticles.