Biosintesis Nanopartikel Perak dengan Ektrak Air Daun Mobe (Artocarpus lakoocha Roxb) dan Aplikasinya sebagai Antioksidan dan Antibakteri

Abstract

Background: Nanoparticles are particles with a nano size of <100 nm. One material that is used widely as nanoparticles is silver (Ag). The synthesis of silver nanoparticles (AgNPs) itself is generally carried out by conventional methods using solvents that are expensive and not ecofriendly. This encourages a better and environmentally friendly synthesis method, one of which is biosynthesis using plant extracts as a bioreductor. Objective: This study aims to synthesize silver nanoparticles using water extract of mobe leaves (Artocarpus lakoocha Roxb) by microwave irradiation method, characterize silver nanoparticles, test antibacterial activity against bacterial Staphylococcus aureus and Staphylococcus epidermidis and test antioxidant activity using DPPH method. Methods: This study includes the synthesis of silver nanoparticles using microwave irradiation method, characterization of synthesized silver nanoparticles including UV-Vis spectrophotometer, PSA, FT-IR and SEM-EDS, antibacterial activity testing with microdilution method on microplate, and antioxidant activity testing using DPPH method. Results: The results of this research show that silver nanoparticles can be synthesized using water extract of mobe leaves (Artocarpus lakoocha Roxb). Silver nanoparticles were characterized using UV-Vis Spectrophotometer which showed λ = 427 nm with absorbance of 1.2485. FT-IR characterization of silver nanoparticles showed that there was no longer an O-H bond from the phenol group as in the water extract of mobe leaves. The existing groups are C-H from alkane groups, C=O from aldehyde groups, C-O from primary alcohol groups and C=C from aromatic groups. SEM results show the agglomeration of silver nanoparticles. EDS results showed that there was a dominant Ag element (89.6%) at a peak of 3.0 keV. PSA results showed particle size of 66.83 nm. Silver nanoparticles showed antibacterial activity against Staphylococcus aureus and Staphylococcus epidermidis with a minimum inhibitory concentration (MIC) at a concentration of 62.5 μg/mL and a minimum bactericidal concentration (MBC) on Staphylococcus aureus and Staphylococcus epidermidis of 125 μg/mL respectively. The IC50 value of mobe leaf water extract is 163.0246±1.05 μg/mL indicating that antioxidant activity is weak and that of silver nanoparticles is 390.9282±2.37 μg /mL indicating that antioxidant activity is very weak. Conclusion: The results showed that silver nanoparticles can be synthesized using the bioreductor of mobe leaf (Artocarpus lakoocha Roxb) water extract, have antibacterial activity against Staphylococcus aureus and Staphylococcus epidermidis and have weak antioxidant activity.