Penentuan Total Fenol, Total Flavonoid dan Aktivitas Antioksidan dengan Metode ABTS Ekstrak Etanol Daun Sandoricum koetjape (Burm.f.) Merr

Abstract

Background: Sandoricum koetjape leaves from the Melliaceae family contain active substances such as flavonoids, tannins, phenols and other secondary metabolites that can play an active role as antibacterial, antimicrobial, antioxidant, anti-inflammatory, anticholesterol, antifungal and anticancer. Objective: To determine the levels of phenols, flavonoids, and antioxidant activity using the ABTS method from the ethanol extract of Sandoricum koetjape (Burm.f.) Merr leaves. Method: Powdered simplisia was macerated with 96% ethanol solvent and concentrated using a rotary evaporator. Then the ethanol extract of Sandoricum koetjape leaves was tested for total phenol content by colorimetric method in UVVis spectrophotometry using Folin-Ciocalteu reagent with gallic acid standard, total flavonoid content was carried out by adding AlCl3 reagent with quercetin standard, and antioxidant activity test against ABTS at 731 nm wavelength using UV-Vis spectrophotometer. Results: Phytochemical screening of Sandoricum koetjape leaves showed the presence of flavonoids, glycosides, saponins, tannins and steroids/triterpenoids. The results showed that the levels of total phenols and total flavonoids contained in the ethanol extract of Sandoricum koetjape leaves were 21,8067 mg GAE/g sample and 10,4821 mg QE/g sample, respectively, and the antioxidant activity test of the ethanol extract of Sandoricum koetjape leaves using the ABTS (2,2'- azino-bis(3- ethylbenz-thiazoline-6-sulfonic acid) method showed an IC50 value of 66,2144±0,0104 μg/ml with strong antioxidant activity category. Conclusion: The results concluded that the ethanol extract of Sandoricum koetjape leaves has high phenol, flavonoid, and antioxidant content.