Pengaruh Variasi ZPT Paclobutrasol dalam Media ½ Murashige dan Skoog (Ms) dan Lama Penyimpanan terhadap Viabilitas Biji Sintetik Chrysanthemum Sp.

Abstract

The distribution of tissue tubers such as Chrysanthemum sp. often encounters obstacles, especially in the problem of its viability, so the manufacture of synthetic seeds is a solution to overcome the problem. The study aimed to determine the combination of paclobutrasol concentration in ½ Murashige and Skoog (MS) medium also the storage time to produced the best synthetic seed of Chrysanthemum sp. callus. Chrysanthemum sp. callus was obtained from UPT. Horticulture Governing Board Medan Johor. Chrysanthemum sp. synthetic seeds are made by encapsulating callus in ½ MS medium with several concentrations of paclobutrazol which were 0, 1, 2, 3, 4, and 5 ppm. In terms of RAL Factorial, the synthetic seeds produced were combined by storage periods of 0, 1, 2, 3, and 4 weeks to determine the viability of the seeds produced with the observed data, namely callus viability, callus growth time, percentage of growth power and callus penetration, browning percentage, percentage of contamination, wet weight and dry weight of callus. The results of all the observation parameters showed that the combination of paclobutrasol treatment in ½ MS medium and storage time is significantly different from the growth time of Chrysanthemum sp. callus, but there is no significant difference in wet weight and dry weight of calluses. The concentration of paclobutrasol of 5 ppm and the storage period of 4 weeks (P5L4) is able to delay the growth of synthetic seeds of Chrysanthemum sp. with 100% viability and could grow by an average day of 86.33. The percentage of synthetic seed growth power of Chrysanthemum sp. is 97.77%, the percentage of seed permeability is 3.33%, the percentage of contamination is 2.22% and the percentage of browning is 3.33%. Increasing concentrations of paclobutrasol in all storage tended to gain the wet weight and dry weight of the callus while longer storage tended to decrease the wet weight and dry weight of the callus when compare to no storage time