| dc.description.abstract | The distribution of tissue tubers such as Chrysanthemum sp. often encounters
obstacles, especially in the problem of its viability, so the manufacture of synthetic
seeds is a solution to overcome the problem. The study aimed to determine the
combination of paclobutrasol concentration in ½ Murashige and Skoog (MS) medium
also the storage time to produced the best synthetic seed of Chrysanthemum sp. callus.
Chrysanthemum sp. callus was obtained from UPT. Horticulture Governing Board
Medan Johor. Chrysanthemum sp. synthetic seeds are made by encapsulating callus
in ½ MS medium with several concentrations of paclobutrazol which were 0, 1, 2, 3,
4, and 5 ppm. In terms of RAL Factorial, the synthetic seeds produced were combined
by storage periods of 0, 1, 2, 3, and 4 weeks to determine the viability of the seeds
produced with the observed data, namely callus viability, callus growth time,
percentage of growth power and callus penetration, browning percentage, percentage
of contamination, wet weight and dry weight of callus. The results of all the
observation parameters showed that the combination of paclobutrasol treatment in ½
MS medium and storage time is significantly different from the growth time of
Chrysanthemum sp. callus, but there is no significant difference in wet weight and dry
weight of calluses. The concentration of paclobutrasol of 5 ppm and the storage period
of 4 weeks (P5L4) is able to delay the growth of synthetic seeds of Chrysanthemum sp.
with 100% viability and could grow by an average day of 86.33. The percentage of
synthetic seed growth power of Chrysanthemum sp. is 97.77%, the percentage of seed
permeability is 3.33%, the percentage of contamination is 2.22% and the percentage
of browning is 3.33%. Increasing concentrations of paclobutrasol in all storage tended
to gain the wet weight and dry weight of the callus while longer storage tended to
decrease the wet weight and dry weight of the callus when compare to no storage time | en_US |
