Aktivitas Antibakteri dan Mekanisme Kerja serta Analisis Kandungan Benzopiren secara Spektrofotometri Uv-Vis dari Asap Cair Cocos nucifera L. Grade 1

Abstract

Liquid smoke is a product that can be obtained industrially from coconut shell (endocarps) through a pyrolysis process at 400°C for 2.5 hours and redistillation to obtain grade 1 liquid smoke. Coconut shell (endocarp) liquid smoke contains chemical compounds, namely phenol, which have been researched to act as a disinfectant. which has broad spectrum antibacterial activity against gram-positive and gram-negative bacteria. This study aims to determine the potential of cocos nucifera L. liquid smoke as an antibacterial against Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA), analysis of benzopyrene content. The concentrations used are 100%, 80%, 60%, 40%, 35%, 30%, 20%, 15%, 10%, 7%, 5%. As a positive control used was Tetracycline 25ppm. The research results show that liquid smoke has the potential to inhibit the growth of both bacteria. The effective concentration for inhibiting the growth of S. aureus bacteria is at a concentration of 40%, while MRSA is at a concentration of 80%. The absorbance values for nucleic acids and proteins were 0.243±0.025 at λ260 nm and 0.393±0.011 at λ280 nm against S. aureus bacteria, while for MRSA bacteria the average absorbance was 0.226±0.007 at λ260 nm and 0.388±0.005 at λ280 nm. Ca2+ and K+ ion leakage was 7.7661 ± 0.0002 ppm and 7.1837 ± 0.0001 ppm in S. aureus, while MRSA was 7.6465 ± 0.0002 ppm and 7.0730 ± 0.0002 ppm. Staphylococcus aureus has a higher sensitivity to liquid smoke Cocos nucifera L. grade 1 than MRSA bacteria. Grade 1 liquid smoke contained benzopyrene 2,07μg/kg which is does not exceed maximum requirements of The Joint FAO/WHO Expert Committee on Food Additives (JEFTA) which is far from 10 μg/kg sample.