| dc.description.abstract | Contamination of detergent containing linear alkylbenzene sulfonate (LAS) waste
could occur due to the absence of a standard system regarding waste management,
especially from laundry businesses. This research aims to obtain biosurfactantproducing
bacterial isolates from water contaminated with detergent waste to degrade
LAS. The parameters observed were the screening for biosurfactant activity, growth of
isolates, biosurfactant concentration, and and the residue of LAS. Isolation was
carried out on selective Bushnell-Haas Agar (BHA) medium which was added with
100 ppm LAS as the only carbon source. The total of obtained 16 bacterial isolates
capable of producing biosurfactants resulted in various IE24 values. Screening for
biosurfactant activity in the form of Emulsification Index (IE24), 6 isolates were
selected to be tested further, they were BSS 15 at 48.5; BSS 08 at 43.8; BSP 20 at 39
.6; BSP 01 at 36.4; BSS 10 at 33 and BSS 13 at 29. The highest cell population was
shown by isolate BSS 15 with total count was 16 x 109 CFU/ml. The Biosurfactant
production by the isolates was performed in Bushnell-Haas Broth (BHB), with the
highest result were concentration in isolate BSS 15 day 5 with a concentration value
of 140.3 ppm; isolate BSS 08 with a value of 130.1 ppm; isolate BSP 13 with a value
of 119.7 ppm and isolate BSS 10 with a value of 110.7 ppm. Furthermore, tests were
carried out on the ability of bacteria to degrade LAS surfactant with the best result
shown by isolates BSS 15 with the reduction up to 88.2% while the lowest one was
shown by isolates BSS 10 with the reduction value was 65.9%. This shows that the BSS
15 isolate has the potential to degrade LAS surfactant because it shows the best results
in terms of biosurfactant activity, bacterial growth, biosurfactant production and LAS
decomposition so that it can be developed as a bioremediation agent. | en_US |
