Seleksi Jamur Endofit Penghasil Lipase dari Daun Kelapa Sawit (Elaeis Guineensis Jacq.)

Abstract

The utilization of endophytic fungi as microorganisms producing extracellular enzymes such as lipase is currently being developed in line with increasing enzyme needs, especially in the industrial field. This research aims to obtain lipase-producing endophytic fungi isolates from oil palm leaves, and identify of potential species as lipolitic producer. Endophytic fungi were isolated from oil palm Medan Selayang District, then the isolates were purified and characterized macroscopically and microscopically. Qualitative selection for lipase-producing was performed by cultivated fungal isolates in Lipid Bromocresol Purple (LBcP) media with olive oil as a substrate. Quantitatively, lipolitc activity were determine by enzyme spesific activity. The potential lipase-producing fungal isolates were identified molecularly using ITS1F and ITS4R primers. The isolation results obtained 12 endophytic fungal isolates. All the fungal isolates had varied of morphological colonies, and hypae microscopically. Eight isolates had asexual spores and 6 isolates had insulated hyphae, but none had clamp connections. A total of 4 fungal isolates have lipolytic activity based on color change in LBcP medium around the fungal colony from purple to yellow. The endophytic fungal isolate with the highest lipolytic ability was DS 01 with an enzyme activity value of 1.111 U/mL, protein content of 2.91 mg/ml, and specific activity of 0.43 U/mg. Molecular identification of DS 01 showed that isolate was closely related to Aspergillus aculeatinus with a similarity percentage of 99.47%.