Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Buah Murbei Merah (Morus Rubra L.)

Abstract

Isolation and identification of flavonoid compounds from red mulberry fruit (Morus rubra L.) has been carried out through several stages, namely maceration extraction with methanol solvent, dissolving with distilled water, fractionation with ethyl acetate solvent, fractionation with n-hexane solvent until a methanol layer is obtained and then evaporated. until all the methanol has evaporated. The concentrated methanol extract was then explained by TLC, then separated by column chromatography with silica gel as the stationary phase and chloroform: ethyl acetate (90:10; 80:20; 70:30; 60:40; 50:50; 40:60; 30: 70; 20:80; 10:90) v/v as mobile phase. The second fraction was chosen to be purified by preparative TLC with silica gel as the stationary phase and chloroform: ethyl acetate (70:30) v/v as the mobile phase. The pure compound obtained was a yellow amorphous solid of 7 mg with a value of Rf = 0.55. Next, the compounds obtained were explained using a UV-Visible Spectrophotometer with the results obtained in 2 bands, namely the first band with a wavelength of 327 nm with an absorption of 0.35822 and the second band with a wavelength of 264 with an absorption of 0.36997, Infrared Spectrophotometer Analysis (FT-IR) with the results obtained that there are functional groups O-H Stretching, C-H Stretching, C=O ketone Stretching, C=C aromatic Stretching, C-H sp3 Bending, C-O Stretching, C-O-C Stretching, C-H sp2 Bending out-of-plane, with successive wave numbers 3425 .58 cm-1, 2956.87 cm-1, 1725.29 cm-1, 1598.99 cm- 1, 1381.03 cm-1, 1286.52 cm-1, 1122.52 cm-1 , 742,59 cm-1. and Proton Nuclear Magnetic Resonance Spectrophotometer (1H-NMR) analysis with the results obtained of the presence of H-2, H-6, H-2', H-6', OCH3 protons. Spectroscopic data shows that the isolated compound is a flavonoid compound thought to be in the isoflavone group.