Pengaruh Kombinasi Thidiazuron (TDZ) dan Casein Hydrolysate terhadap Multiplikasi Tunas Pisang Barangan Merah (Musa acuminata Linn.) Secara In Vitro

Abstract

The aim of research was to determine the effect and see the interaction between the concentration of Thidiazuron (TDZ) and Casein Hydrolysate on the multiplication of barangan red banana buds (Musa acuminata Linn) in vitro. This research was conducted at the Tissue Culture Laboratory, Alifa Agricultural Research Center (ALIFA-ARC) Medan. This research will be conducted from November 2023 to February 2024. This study used the Complete Randomized Design (CRD) method with two factors. The first factor is the concentration of casein hydrolysate (C): C0 (0 mg / l), C1 (100 mg / l), C2 (200 mg / l) and C3 (300 mg / l). The second factor is the concentration of thidiazuron (T): T0 (0 mg / l), T1 (0.2 mg / l), T2 (0.4 mg / l) and T3 (0.6 mg / l). The observed variables were the percentage of live explants, the number of shoots, the length of shoots, the number of roots, and the length of roots. The results showed that thidiazuron treatment had a significant effect on shoot multiplication. Casein hydrolysate treatment had no significant effect on shoot multiplication. The combination of thidiazuron and casein hydrolysate treatment had no significant effect on shoot multiplication