Potensi Gel Kitosan Oligosakarida dalam Menghambat Pertumbuhan Bakteri Fusobacterium nucleatum (In Vitro)

Abstract

Fusobacterium nucleatum is one of the common pathogenic bacteria causing periodontal disease in the oral environment. Scaling-root planing is the cornerstone of non-surgical periodontal therapy in removing biofilm. However, this therapy has limitations in accessing deep and tortuous pockets, and there is still the problem of microbial repopulation and re-infection after scaling-root planing, especially in cases where instrumentation is inadequate. Therefore, a combination with antibacterial drugs is usually required to selectively inhibit or kill microorganisms. Chitosan oligosaccharide is one of the primary degradation products of the natural material chitosan, known for its biocompatible, biodegradable, and antibacterial properties. The purpose of this study was to determine the potential of chitosan oligosaccharide gel in inhibiting the growth of Fusobacterium nucleatum bacteria. This type of research is laboratory experimental research with posttest control group design. The samples of this study were F. nucleatum bacteria isolated, incubated, and cultured on Brucella Agar Sheep Blood 5%. This study was tested using the Kirby-Bauer method with repetition four times. The results indicate that chitosan oligosaccharide gel at concentrations of 12.5%, 20%, and 25% demonstrated strong inhibitory activity (11–20 mm) against F. nucleatum, whereas concentrations of 1.5%, 3%, and 6.25% showed no inhibitory zone. The conclusion of this study is that 12.5%, 20%, and 25% oligosaccharide chitosan gels have the potential to inhibit the growth of F. nucleatum bacteria, while concentrations of 1.5%, 3%, 6.25% have no potential.