Uji Daya Hambat Chip Kitosan Oligosakarida terhadap Pertumbuhan Bakteri Porphyromonas gingivalis dan Fusobacterium nucleatum (In Vitro)

Abstract

Porphyromonas gingivalis and Fusobacterium nucleatum are bacteria that play a role in the development of periodontal disease. Scaling and root planing are the gold standard in periodontal treatment, but their effectiveness is limited due to their inability to reach areas with deep pockets. This treatment can be combined with the application of local antibiotics. Chitosan oligosaccharides are one of the natural materials that are biocompatible and non-toxic, and have antibacterial properties. The aim of this research is to determine the inhibitory activity of chitosan oligosaccharide chips against the growth of P. gingivalis and F. nucleatum bacteria. The type of this research is a laboratory experimental study with a posttest control group design. The samples in this study are the bacteria P. gingivalis and F. nucleatum, which were isolated, incubated, and cultured on 5% Brucella Agar Sheep Blood. This study used the modified Kirby-Bauer method. The results of this study show strong inhibitory activity of 7.5% and 10% chitosan oligosaccharide chips against the growth of P. gingivalis bacteria, and at a concentration of 10%, they exhibit strong inhibitory activity against the growth of F. nucleatum bacteria. The conclusion of this study is that chitosan oligosaccharide chips are effective in inhibiting the growth of P. gingivalis and F. nucleatum bacteria.