Optimalisasi Ekstrak Daun Sirih Cina (Peperomia Pellucida L.) Terhadap Histologi Paru-Paru Mencit (Mus Musculus L.) Yang Dipapar Asap Rokok

Abstract

Cigarette smoke contains various harmful substances, including free radicals that smoke can lead to oxidative stress, damaging lung cells, increasing the risk of inflammation, and impairing of the lung function. The use of natural antioxidants can be used to repair the damage. Chinese betel leaf (Peperomia pellucida L.) is known as a source of flavonoids with high antioxidant properties. This research method used a completely randomized design (CRD) consisting of 6 treatments each, which were the control without treatment; the exposure by cigarette smoke only; the exposure to cigarette smoke and being given vitamin C; the exposure to cigarette smoke and given Peperomia leaf extract at doses of 50, 70 and 90mg/Kg of body weight. This research used samples of 24 male mice (Mus musculus L.) as testing animals. The Observations made were the morphology such as the weight, the color, and the surface of the lung and also the histology (alveolar membrane, alveolar lumen, and inter-alveolar connections). The results show the most optimal of the Chinese betel leaf extract at a dose of 90mg/KgBB can improve the morphology of the lungs of mice, namely the color, weight and surface of the lungs of mice. The administration of the chinese betel leaf extract at doses of 50mg/KgBB and 70mg/KgBB is given less optimal in significantly improving the histology of mice lungs. The chinese betel leaf extract at a dose of 90mg/KgBB is the most optimal dose that significantly improve the histology of mice lungs by reducing the damage to the alveolus membrane, the alveolus lumen, and the relationship between alveoli due to exposure to cigarette smoke.